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Ultraviolet Spectrum (UV) Testing

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Ultraviolet Spectrum (UV) Testing

Ultraviolet absorption method can be used to determine the composition and content of substances according to the absorption of different wavelengths of ultraviolet light. Ultraviolet spectroscopy can be applied to the determination of unsaturated organic substances, especially conjugated systems, to infer the skeleton structure of unknown substances, which is very important for understanding the material world from the molecular level. As a professional reliability third-party testing organization, T,C&A Lab's Ultraviolet Spectrum Testing Laboratory can perform a full range of analysis services according to ISO, ASTM and other standards as well as industry requirements. Welcome to contact our experts for consultation.

Analysis and testing capabilities of ultraviolet spectrum

  • Compound identification

The ultraviolet spectrum can be used to deduce whether there are conjugated structure systems in the molecular skeleton of organic compounds, such as C=C-C=C, C=C-C=O, benzene ring and so on.

  • Purity inspection

If the organic compound has no obvious absorption peak in the UV-visible region, and the impurities have a strong absorption in the UV region, the UV spectrum can be used to test the purity of the compound.

  • Isomer determination

For the determination of isomers, λmax can be calculated through empirical rules and compared with the measured value to confirm which isomer the compound is.

  • Determination of steric hindrance

The steric hindrance will affect the coplanar nature of the conjugated system. When the chromogenic groups of the conjugated system are approximately in the same plane and the two chromogenic groups have greater resonance, the steric hindrance effect is small.

  • Determination of hydrogen bond strength

When solvent molecules associate with solute molecules to form hydrogen bonds, they have a greater impact on the UV spectrum of solute molecules.

  • Quantitative analysis

Analysis methods include Lambert-Beer law, comparison method and standard curve method.

Friendly tip: UV spectrum is only an auxiliary analysis method, and other analysis methods, such as infrared, nuclear magnetic resonance, EPR and other comprehensive methods are needed to analyze the tested objects in order to get accurate data.

Our test cases include, but are not limited to

  • Quantitative determination of soluble lignin and furfural in lignocellulosic pre-hydrolyzed solution by ultraviolet spectroscopy.

  • UV spectrum analysis to monitor water quality.

  • Monitoring of aspirin and salicylic acid in aspirin synthesis system by ultraviolet spectroscopy.

  • Determination of caffeine in Cola beverage by ultraviolet spectroscopy.

Sample requirements

  • The concentration of the sample solution must be appropriate, and it must be clear and transparent, and there must be no bubbles or suspended matter.

  • Solid sample > 0.2g, liquid sample > 2mL.

Standards we test to

  • ASTM D2008, ASTM E275, ASTM D8071, ASTM D8267, ASTM D2269, ASTM D5510, ASTM D1840, ASTM D5156

  • ISO/TS 17466, ISO 11264, ISO 10640, ISO 3656, ISO 22478, ISO 20179

In addition, the experts in our Ultraviolet Spectrum Testing Laboratory also provide a variety of custom services as your needs and requirements. Let's discuss the custom services with our experts for free.

Instruments and data

Ultraviolet Spectrum (UV) Testing
UV Testing


  1. Satheeshkumar, E.; et al. One-step solution processing of Ag, Au and Pd@ MXene hybrids for SERS. Scientific reports 6 (2016): 32049.

Note: the service is for research use only and not intended for any clinical use.


T,C& A LAB is an independent lab providing quality or custom testing, characterization and analysis of a variety of materials. Our engaged experts are ready to help you.

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